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Cold-water lime pasteurisation

Straw soaked overnight in a high-pH hydrated lime bath; no heat needed.

pH 12 limehydrated lime, low magnesium
Plate 9 of 16Cold-water lime pasteurisation, drawn for All the Tek.

What it is

Lime raises the pH of the soaking water to 12 or 13, which kills most competitors, and oyster mycelium is then added to the drained straw1. Cornell’s guide stresses using hydrated lime with a magnesium content under 10%1.

As a starting point the guide adds 0.35 to 0.5 lb of lime per gallon of water, checks the pH with test strips and soaks the straw for 16 to 18 hours, no longer1.

In a Northeast SARE farm trial, straw soaked 16 hours at pH 12–13 gave on average 12% more mushrooms than hot-water pasteurisation, and fully contaminated bags were much more common with fermentation and wood-ash treatments than with lime or heat2. That is one farm’s trial; Cornell’s own table lists lime as "moderately reliable"1.

What it suits

Oyster mushrooms on straw1.

Species notes are on the species page; for the mushrooms themselves, see Ohio Gourmet Mushrooms.

Equipment

  • Hydrated lime with magnesium under 10%1
  • A barrel or tote big enough to submerge the straw
  • pH test strips
  • Gloves and eye protection
  • Mesh bags or a basket to lift the straw out
  • Oyster spawn and bags or buckets

Time to first harvest

About 3–4 weeks to colonise, then fruiting. In the SARE trial, lime-treated bags incubated for 3 to 4 weeks before moving to fruit2. Cornell gives 14 to 28 days of incubation for oysters on straw1.

Contamination and other risks

  • Lime with too much magnesium, which Cornell calls critical to avoid1.
  • Soaking longer than 18 hours1.
  • The same green moulds and insects as hot-water straw31.

Steps, in overview

  1. Mix. Stir hydrated lime into water at roughly 0.35–0.5 lb per gallon and check that the pH reads 12 or more1.
  2. Soak. Submerge the straw for 16–18 hours1.
  3. Drain. Lift out and drain; the SARE trial drained for 20 minutes2.
  4. Spawn and pack. Mix in oyster spawn and pack bags or buckets firmly.
  5. Neutralise the water. Bring the pH down with a pH-down product before disposal1.

Common mistakes

  • Buying dolomitic or garden lime instead of low-magnesium hydrated lime1.
  • Skipping the pH check1.
  • Pouring the high-pH water out untreated1.

Safety

Hydrated lime (calcium hydroxide) irritates and can burn the eyes and skin, and irritates the airways4. Wear gloves and eye protection, avoid breathing the dust when mixing, and rinse eyes or skin straight away if it gets on you4. Cornell also says to wear gloves when handling the straw or water1.

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Sources

  1. Methods of Commercial Mushroom Cultivation in the Northeastern United States. Willie Crosby (Fungi Ally) and Steve Gabriel, Cornell Small Farms Program, 2019 (Northeast SARE). Extension publication, checked 26 October 2026.
  2. Alternative growing practices for oyster mushroom cultivation in the Northeast (FNE15-825). Willie Crosby, Fungi Ally, Northeast SARE farmer grant final report. Farm trial report, checked 26 October 2026.
  3. D.I.Y. FunGuide: Grow Your Own Oyster Mushrooms at Home. Hameed, Cotter, Liao and Hsu, UF/IFAS Extension (EDIS SS662). Extension publication, checked 26 October 2026.
  4. NIOSH Pocket Guide to Chemical Hazards: Calcium hydroxide. CDC / NIOSH. Government, checked 26 October 2026.